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  Specialist - Protein Sciences  

  Aurigene Pharmaceutical Services Limited

  Permanent - Hyderabad

  Posted: 25/03/2026

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  Protein Sciences - Roles & ResponsibilitesDesign & execute end-to-end... Read More

  Protein Sciences - Roles & Responsibilites

  
  

  Design & execute end-to-end protein purification workflows (ab initio or SOP-based) for drug discovery projects from expression to crystallization-ready samples

  
  

  Optimize recombinant protein expression across E. coli, yeast, insect (Sf9), and mammalian (HEK/CHO) systems using affinity, IEX, SEC, and HIC chromatography (AKTA/FPLC)

  
  

  Support membrane protein purification (detergent screening, nanodiscs, solubilization) and prepare high-quality samples for crystallization/cryo-EM

  
  

  Perform QC analytics: SDS-PAGE, Western blot, SEC-HPLC, DLS, CD, endotoxin testing to ensure protein fit-for-purpose for screening or structural studies

  
  

  Analyze and interpret assay data using GraphPad Prism, UNICORN, or Empower; present findings to drive molecule progression decisions

  
  

  Maintain ALCOA+ compliant eLN documentation, adhere to GLP/GMP SOPs, and ensure 100% lab safety & quality compliance

  
  

  Collaborate with structural biology, biophysics, and assay teams; contribute to process improvement, knowledge sharing, and platform innovation

  
  

  Requirements: M.Sc/M.Tech in Biotechnology/Biochemistry, 9-14 years' CRO/biopharma experience, hands-on AKTA/chromatography expertise, SnapGene proficiency

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• A

  Scientist - Molecular Biology  

  Aurigene Pharmaceutical Services Limited

  Permanent - Bangalore

  Posted: 25/03/2026

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  About the RoleWe are seeking a self-driven Scientist to lead and deliv... Read More

  About the Role

  We are seeking a self-driven Scientist to lead and deliver end-to-end molecular biology projects in our NBE Services portfolio. You will design and execute strategies for engineered antibodies, enzymes, structural proteins, and mutant strains - supporting global drug discovery programs in a fast-paced CRO environment.

  
  

  Key Responsibilities

  Design and execute molecular biology workflows for production of engineered antibodies, enzymes, structural/reagent proteins, and mutant strainsLead gene synthesis, cloning, vector design/swapping, antibody gene sequencing, and site-directed mutagenesis projectsGenerate CRISPR-based mutants in prokaryotic systems; perform sequence data analysis and validationExecute mRNA isolation, cDNA synthesis, and gene expression analysis using Real-Time PCRSupport HCP/HCD estimation, data analysis, and protein engineering initiativesOversee plasmid production and chromatographic purification (affinity, IEX, SEC)Manage microbial, yeast, and fungal strain engineering, including cell bank preparation and characterizationOperate and optimize automation/high-throughput platforms (liquid handlers, robotic systems)Mentor and lead the molecular biology team; drive project planning, execution, and client communicationPrepare technical reports, presentations, project proposals, and SOPs with minimal reviewEnsure 100% compliance with GLP/GMP, ALCOA+ data integrity, and lab safety standards

  
  

  Must-Have Qualifications

  6-10 years of hands-on industrial or advanced academic experience in molecular biology R&DExpertise in:

  • Cloning (In-Fusion, Gibson, TOPO, ligation-based)

  • Vector design, gene synthesis, mutagenesis, antibody sequencing

  • CRISPR-based editing in prokaryotes

  • RT-qPCR, mRNA/cDNA workflows, sequence analysis

  • Mammalian/microbial cell culture and strain engineering

  • SnapGene, Sequencher, Benchling, or equivalent bioinformatics tools

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